Ethanolic Extract of Garcinia kola (Heckel) Seed Possesses Antiplasmodial Properties against Plasmodium berghei

Main Article Content

A. I. Airaodion
J. A. Ekenjoku
K. O. Ngwogu
A. C. Ngwogu

Abstract

Background: There is a considerable increase in mortality caused by malaria due to the rapid spread of drug-resistant strains of Plasmodium falciparum and Plasmodium berghei. The parasites have developed resistance to orthodox drugs over the years, thus the need for herbal remedy.

Aim: This study therefore sought to investigate the antiplasmodial properties of ethanolic extract of Garcinia kola seed against Plasmodium berghei in infected Swiss albino mice.

Methods: G. kola seeds were air-dried and ground to fine powder using a blender. The powder was extracted using ethanol as the solvent. Sixty Swiss albino mice were divided into six groups of ten each. Each mouse in groups 2 to 6 was inoculated intraperitoneally with infected blood suspension containing about 1x107Plasmodium berghei parasitized red blood cells on day zero while those in group 1 were not infected and this served as the normal control group. Animals in group 2 were administered 0.2 mL normal saline, those in group 3 were administered Chloroquine diphosphate at 5 mg/kg body weight, those in groups 4, 5 and 6 were administered 100, 200 and 400 mg/kg of G. kola seed extract respectively. All treatments were orally done twelve hourly for five consecutive days from when parasites were first seen in the infected animal blood. Parasitaemia count and packed cell volume were done using standard methods.

Results: G. kola extract exhibited antimalarial properties especially at doses of 200 and 400 mg/kg and the results were not different from that of chloroquine.

Conclusion: The result of this study showed that ethanolic extract of G. kola seed possesses antiplasmodial properties against P. berghei in a dose-dependent manner. Maximum antimalarial potency of plant extracts and standard antimalarial drugs can be derived when dosage is completed.

Keywords:
Antiplasmodial properties, Garcinia kola, malaria, Plasmodium berghei.

Article Details

How to Cite
Airaodion, A. I., Ekenjoku, J. A., Ngwogu, K. O., & Ngwogu, A. C. (2020). Ethanolic Extract of Garcinia kola (Heckel) Seed Possesses Antiplasmodial Properties against Plasmodium berghei. Asian Journal of Medical Principles and Clinical Practice, 3(1), 33-39. Retrieved from http://journalajmpcp.com/index.php/AJMPCP/article/view/30110
Section
Original Research Article

References

Hutchson J, Dalziel JM. Flora of West Tropical Africa. 2nd Ed. H.M.S.O London. 1956;8(11):295.

Plowden CC. A manual of plants names. 3rd ed. London. George Ltd. 1972;239.

Burkhill HM. The useful plants of west tropical Africa – A digital library of scholarly resources from and about Africa; 2011.
Available:http://www.aluka.org

Ofusori DA. Ayoka AO, Adelekun AE, Falana BA, Adeeyo OA, Ajeigbe KO, Yusuf UA. Microanatomical effects of ethanolic extract of Garcinia kola on the lungs of swiss albino mice. The Internet Journal of Pulmonary Medicine. 2008;10:1.

Aniche GN, Uwakwe GU. Potential use of Garcinia kola as a hop substitute in lager beer brewing. World Journal of Microbiology and Biotechnology. 1990;(6):323–486.

Bnouham M, Abderrahim Z, Hassane M, Abdelhafid T, Abdelkhaleq L. Medicinal plants with potential antidiabetic activity – A review of ten years of herbal medicine research (1990-2000). International journal of Diabetes & Metabolism. 2006;12(14):1–25.

Airaodion AI, Ngwogu AC, Ekenjoku JA, Ngwogu KO. Hepatoprotective potency of ethanolic extract of Garcinia kola (Heckel) seed against acute ethanol-induced oxidative stress in Wistar rats. International Research Journal of Gastroenterology and Hepatology. 2020;3(2):1-10.

Anyensu ES. Medical Plants of West Africa. Reference Publication Incoporated; Algonac, MI. 1978;162.

Iwu MM. Pharmacognotical Profile of selected medicinal plants. In: Handbook of African medicinal plants. CRC press, Boca Raton, Florida. 1993;183.

Iwu MM. In plant flavonoids in Biology and Medicine V, Cody E. Middleton and JB Harbone eds. Ala R. Liss. New York. 1986;485.

Ibironke GF, Olaleye SB, Balogun O, Aremu DA. Antiucerogenic effect of diets containing seeds of Garcinia kola (Hekel) Phytotherapy Research. 1997;4(11):312-313.

Igboko AO. Phytochemical Studies in Garcinia kola. Hekel. Msc. Thesis, University of Nigeria Nsukka, Nigeria, (Unpublished); 1983.

Braide VD, Vittrotio G. Histological alterations by a diet containing seeds of Garcinia kola: Effects on liver, kidney and intestine in the rat. In: Gedenbaurs Morphology. Jahrb, Leipzig. 1989;1334:95-101.

Maduinyi I. Biochemical and pharmiocol studies of the active principles of the seeds of Garcinia kola. M.Sc Thesis, University of Nigeria Nsukka, Nigeria; 1983.

Eka OU. Studies in the feasibility of replacing hop by other bittering substances in brewing. Nigerian Journal of Microbial. 1984;4(1-2):43-51.

Jena BS, Jayaprakasha GK, Singh RP, Sakariah KK. Chemistry and biochemistry of hydroxycitric acid from Garcinia. Journal of Agriculture, Food and Chemistry. 2002;50(1):10-22.

Masullo M, Bassarello C, Suzuki H, Pizza C, Piacente S. Polyisoprenylated benzophenones and an unusual polyisoprenylated tetracyclic xanthone from the fruits of Garcinia cambogia. Journal of Agriculture, Food and Chemical Toxicology. 2008;56(13):5205-5210.

Koshy AS, Anila L, Vijayalakshmi NR. Flavonoids from Garcinia cambogia lower lipid levels in hypercholesterolemic rats. Food and Chemical Toxicology. 2001;72(3):289-294.

Airaodion AI, Airaodion EO,Ekenjoku JA, Ogbuagu EO, Ogbuagu U. Antiplasmodial potency of ethanolic leaf extract of Carica papaya against Plasmodium berghei in infected swiss albino mice. Asian Journal of Medical Principles and Clinical Practice. 2019;2(2):1-8.

Miller LH, Good MF, Milon G. Malaria pathogenesis. Science. 1994;264:1878-1994.

WHO. The world health report-changing history. Geneva: World Health Organization. 2004;96.

Peters W. Antimalarial drug resistance: An increasing problem. Br Med Bull. 1982;32: 187-192.

Akuodor GC, Idris UI. Anti-nociceptive, anti-inflammatory and antipyretic effect of the methanolic extract of Bombax buonopozense seeds in rats and mice. African Journal of Biotechnology. 2011;10: 3191-3196.

Ryley J, Peters W. The antimalarial activity of some quinoline esters. Ann Trop Med Parasitology. 1995;84(22):209–2.

Airaodion AI, Airaodion EO, Ogbuagu U, Ekenjoku JA, Ogbuagu EO. Antimalarial efficacy of ethanolic seed extract of Vernonia amygdalina against Plasmodium berghei in infected swiss albino mice. International Journal of Bio-Science and Bio-Technology. 2019;11(8): 68-76.

Langhorne J, Quin SJ, Sanni LA. Mouse models of blood-stage malaria infections: Immune responses and cytokines involved in protection and pathology. In: Perlmann P, Troye-Blomberg M, editor. Malaria Immunology. Stockholm: Karger Publisher. 2002;204–228.

Bantie L, Assefa S, Teklehaimanot T, Engidawork E. In vivo antimalarial activity of the crude seed extract and solvent fractions of Croton macrostachyus Hocsht. (Euphorbiaceae) against Plasmodium berghei in mice. BMC Complement Altern Med. 2014;14:79.

Airaodion AI, Ogbuagu U, Ekenjoku JA, Ogbuagu EO. Comparative assessment of haematopoietic potential of ethanolic extract of Telfairia occidentalis and Talinum triangulare leaves in wistar rats. Acta Scientific Nutritional Health. 2019;3 (10):38-43.

Airaodion AI, Ogbuagu EO, Ekenjoku JA, Ogbuagu U, Airaodion EO. Haematopoietic Potential of ethanolic leaf extract of Talinum triangulare in wistar rats. Asian Journal of Research in Biochemistry. 2019; 5(2):1-7.

Chanphen R, Thebtaranonth Y, Wanauppathamkul S, Yuthavong Y. Antimalarial principles from Artemisia indica. Journal of Natural Products. 1998; 61:1146-1147.

Damian DC, Nweze EI, Onyeke CC). The in vivo anti-plasmodium activity of Garcinia kola Heckel. Journal of Basic Pharmacology and Toxicology. 2017;1(2): 27-31.