An Assessment of Hepato-Protective Activity of Psidium guajava Fruit Extract against Hepatic Injured Rodent Model

Md. Abdullah Hil Baky Rupak

Department of Pharmaceutical Sciences, North South University, Plot # 15, Block # B, Bashundhara R/A, Dhaka, 1229, Bangladesh.

F. M. Sharifuzzaman Shohan

Department of Pharmacy, Primeasia University, Bangladesh.

Juliana Aditi Baroi

Department of Pharmacy, University of Asia Pacific, Farmgate, Dhaka, Bangladesh.

Md. Rahmat Ullah

Department of Pharmacy, University of Asia Pacific, Farmgate, Dhaka, Bangladesh.

Md. Mustafiz Chowdhury

Department of Pharmacy, University of Chittagong, Bangladesh.

Bornila Mehjabin

Department of Pharmacy, State University of Bangladesh, Bangladesh.

Puja Bhowmik

Department of Pharmacy, University of Science & Technology Chittagong, Bangladesh.

Nur-Neasha Dolon

Department of Pharmaceutical Sciences, North South University, Plot # 15, Block # B, Bashundhara R/A, Dhaka, 1229, Bangladesh.

Rafat Tashin *

Department of Pharmaceutical Sciences, North South University, Plot # 15, Block # B, Bashundhara R/A, Dhaka, 1229, Bangladesh.

*Author to whom correspondence should be addressed.


Herbal remedies are the art or practice of using herbs and herbal medicines to maintain health and prevent, treat, or cure sickness. The lipid profiles of Psidium guajava extract were studied in rats. In the case of Serum glutamic pyruvic transaminase (SGPT) and serum glutamic-oxaloacetic transaminase (SGOT), only the high dose produced statistically significant (p<0.05) outcomes as compared to the negative control group. At low, medium, and high dosages, creatinine levels decreased statistically significant (p<0.05). However, none of the dosages in case of urea produced statistically significant (p<0.05) effects. Only at high dosages did total cholesterol levels reduce statistically significantly (p<0.05) when compared to the negative control group. Only at high doses did High Density Lipoprotein (HDL) levels improve statistically significantly (p<0.05). High and medium dosages of Low Density Lipoprotein (LDL) significantly (p<0.05) reduce the quantity in the blood. Triglyceride levels were reduced only at high dosages as compared to the negative control group. These discoveries may benefit those suffering from liver disorder.

Keywords: Psidium guajava, SGPT, Herbal medicine, phytopharmacology, cholesterol

How to Cite

Rupak , M. A. H. B., Shohan , F. M. S., Baroi , J. A., Ullah, M. R., Chowdhury , M. M., Mehjabin , B., Bhowmik , P., Dolon , N.-N., & Tashin , R. (2023). An Assessment of Hepato-Protective Activity of Psidium guajava Fruit Extract against Hepatic Injured Rodent Model. Asian Journal of Medical Principles and Clinical Practice, 6(2), 240–245. Retrieved from


Download data is not yet available.


Mokdad AA, Lopez AD, Shahraz S, Lozano R, Mokdad AH, Stanaway J, Murray CJ, Naghavi M. Liver cirrhosis mortality in 187 countries between 1980 and 2010: A systematic analysis. BMC Med. 2014;12:145.

DOI: 10.1186/s12916-014-0145-y PMID: 25242656; PMCID: PMC4169640.

Poli G. Liver damage due to free radicals. Br Med Bull 1993;49(3):604-620.

3.Traverso N, Ricciarelli R, Nitti M, Marengo B, Furfaro AL, Pronzato MA, Marinari UM, Domenicotti C. Role of glutathione in cancer progression and chemoresistance. Oxid Med Cell Longev. 2013;2013:972913.

DOI: 10.1155/2013/972913. Epub 2013 May 20. PMID: 23766865; PMCID: PMC3673338.

Franco R, Cidlowski J Apoptosis and glutathione: beyond an antioxidant. Cell Death Differ 2009;16(10):1303-1314.

Kerksick C, Willoughby D .The Antioxidant Role of Glutathione and N-Acetyl-Cysteine Supplements and Exercise-Induced Oxidative Stress. J Int Soc Sports Nutr. 2005;2(2):38-44.

Kretzschmar M. Regulation of hepatic glutathione metabolism and its role in hepatotoxicity. Exp Toxicol Pathol. 1996;48(5):439-446.

Baroi JA, Hossian MR, Chowdhury MM, Dolon NN, Maliha F, Rupak MA, Lima NN, Ullah MR, Tahsin R. An assessment of anti-hyperlipidemic potentialities of ethanolic extract of hemidesmus indicus in high fat induced rat model. Asian Journal of Food Research and Nutrition. 2023;2(4):323-30.

Yang L, Stöckigt J. Trends for diverse production strategies of plant medicinal alkaloids. Natural product reports 2010;27(10):1469-1479.

Saxena M, Saxena J, Nema R, Singh D, Gupta A. Phytochemistry of medicinal plants. Journal of Pharmacognosy and Phytochemistry. 2013;1(6):168-182

Rupak MA, Chowdhury MM, Shurovi FS, Ferdous J, Tahsin MR, Sarif S, Hasan MM, Chowdhury JA, Kabir S, Chowdhury AA, Aktar F. An Evaluation of Analgesic and Anti-Inflammatory Activity of Ethanolic Extract of Cynodon Dactylon on Stressed Rodent Model. Biomedical Journal of Scientific & Technical Research. 2022;42(3):33550-7.

Mim IJ, Peya FY, Chowdhury MM, Khan TR, Mandal SK, Maliha F, Alam M, Rahman T, Tashin R. An Evaluation of Anti-Diabetic Activity of Ethanolic Extract of Asparagus racemosus in Alloxan Induced Rat Model. Int. J. Adv. Nephrol. Res. 2023;6(1):60-8.

Arbab AH, Parvez MK, Al-Dosari S. et al, Hepatoprotective and antiviral efficacy of Acacia mellifera leaves fractions against hepatitis B virus, BioMed Research International; Article ID 929131, 2015;10.

Al-Qarawi AA, Al-Damegh MA, El-Mougy SA, Hepatoprotective influence of Adansonia digitata Pulp, Journal of Herbs, Spices & Medicinal Plants. 2003;10(3):1-6

Musa E, Badwi EL, Osman EA, Dahab MM, Hepatoprotective and toxicity assessment of Cannabis sativa seed oil in Albino rat, International Journal of Clinical and Biological Sciences. 2012;1:69–76.

Psidium guajava". Germplasm Resources Information Network. Agricultural Research Service, United States Department of Agriculture. Retrieved;2009.

Das M, Goswami S. Antifungal and antibacterial property of guava (Psidium guajava) leaf extract: Role of phytochemicals. Int. J. Health Sci. Res. 2019;9(2):39-45.

Gayathri V, Kiruba D. Preliminary phytochemical analysis of leaf powder extracts of Psidium guajava L. International Journal of Pharmacognosy and Phytochemical Research. 2014;6(2):332-4..

Gupta M, Mazumder UK, Kumar TS, Gomathi P, Kumar RS, Antioxidant and hepatoprotective effects of Bauhinia racemosa against paracetamol and carbon tetrachloride induced liver damage in rats, Iranian Journal of Pharmacology and Therapeutics. 2004;3(1):12-20.

Pradhan S, and Girish C, Hepatoprotective herbal drug, silymarin from experimental pharmacology to clinical medicine, Indian Journal of Medical Research. 2006;124(5):491-504.

Chatterjee T, Medicinal plants with hepatoprotective properties, in Herbal Options, Books and Allied (P) Ltd, Calcutta, India, 3rd edition; 2000;135.

Subramaniam S, Hedayathullah Khan HB, Elumalai N, Sudha Lakshmi SY. Hepatoprotective effect of ethanolic extract of whole plant of Andrographis paniculata against CCl 4-induced hepatotoxicity in rats. Comparative Clinical Pathology. 2015;24:1245-51.

Junejo JA, Gogoi G, Islam J, Rudrapal M, Mondal P, Hazarika H, Zaman K. Exploration of antioxidant, antidiabetic and hepatoprotective activity of Diplazium esculentum-A wild edible plant from North Eastern India. Future Journal of Pharmaceutical Sciences. 2018;4(1):93-101.

Koyagura N, Jamadar MG, Huilgol SV, Nayak N, Yendigeri SM, Shamsuddin M. Antidiabetic and hepatoprotective activities of Tamarindus indica fruit pulp in alloxan induced diabetic rats. International Journal of Pharmacology and Clinical Sciences. 2013;2(2).

Biswas S, Talukdar P, Talapatra SN. Presence of phytochemicals in fruits and leaves of guava (Psidium guajava Linn.) for cancer prevention: A mini review. Journal of Drug Delivery and Therapeutics. 2019;9(4-s):726-9.

Roy R, Roy J, Liya IJ, Ahmed J, Akter A, Basher MA. Antioxidant and hepatoprotective activities of methanolic extract of Fimbristylis miliacea (L.) Vahl. Phytomedicine Plus. 2023;3(2):100449.

Rahmat A, Bakar MF, Hambali Z. The effects of guava (Psidium guajava) consumption on total antioxidant and lipid profile in normal male youth. African Journal of Food, Agriculture, Nutrition and Development. 2006;6(2).